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In some situation , specially in study of bacterial community from plant DNA, chloroplast and mitochondrial organells DNA could be contaminated.
Application of the primer 799f that specify bacterial sequences to exclusion of chloroplast DNA is useful
Organelle DNA : Genes located outside the cell nucleus in the mitochonderia or chloroplast (plastid).,
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For many applications, it is necessary to visualize tissue anatomy. We counterstain with propidium iodide (PI), a nucleic acid binding dye. The result is red fluorescence surrounding each cell in the root. Keeping roots healthy is the best way to guarantee good image
reference
doi: 10.1186/s12870-016-0756-x
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In environment any compound or molecule that persists in nature for long time and resist degradation
Some natural organic molecules can resist microbial decomposition because of certain molecular properties forms
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Glomeromycota are mutualistic symbionts that form arbuscular mycorrhizal (AM) associations intracellularly within the roots of the vast majority of herbaceous plants and tropical trees
The phylum Glo
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Taghinasab M, Imani J, Steffens D, Glaeser SP, Kogel K-H (2018) The root endophytes Trametes versicolor and Piriformospora indica increase grain yield and P content in wheat. Plant and Soil. doi: 10.1007/s11104-018-3624-7.
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